511 Three-dimensional culture of human sensory neurons derived from iPS cells in a pigmented and endothelialized tissue-engineered reconstructed skin enriched with Schwann cells

Communication between the nervous system and melanocytes have long been suspected on basis of their common embryologic origin several forms clinical evidence. Histological ultrastructural studies ultimately confirmed these intimate morphological contacts in normal human skin, which are strikingly exacerbated pathophysiology certain dermatoses hyperpigmented lesions. To date, no vitro model comprises required features to accurately study interactions. build a fully innervated pigmented tissue-engineered model, skin equivalents (SEs) were prepared by co-culturing dermal fibroblasts microvascular endothelial cells with hiPSC-derived sensory neurons into unique porous scaffold made chitosan-cross-linked collagen glycosaminoglycan polymer, subsequently epidermized keratinocytes melanocytes. Human Schwann also added during culture equivalent. analysis that produced well-organised terminally differentiated epidermis containing numerous homogenously distributed basal cell layer. At level, CD31 podoplanin immunostaining demonstrated blood lymphatic adhered proliferated form capillary-like ring structures embedded neo-synthetized extracellular matrix (ECM). Neurofilaments network from iPSC-derived seeded at bottom sponge infiltrated entire native ECM, as shown immunodetection TUJ1. Spanning neurites was not only limited dermis but found within epidermal compartment close contact This advanced 3D integrating seven different types including functional pigmentary may be relevant tool further decipher interactions systems.